control non targeting sirna  (Santa Cruz Biotechnology)

Journal: Clinical Science (London, England : 1979)

Article Title: In vivo inhibition of TDO2 in fibroids results in widespread alteration in the tumor transcriptome

doi: 10.1042/CS20260395

Figure Lengend Snippet: Effects of TDO2 silencing in primary LSMC. Cells were transfected with siTDO2 or control siRNA (siNC) for 96 h, followed by assessment of gene expression by qRT-PCR. Expression levels of TDO2, VDR, MMP11, MMP14, COL11A1, CBX4, LINC02568, LINC01310, LINC02544, LINC02182, and miR-584-5p are shown. Data represent mean ± SEM from four independent experiments ( n = 4). Statistical significance is indicated as * P <0.05 and *** P <0.01.

Article Snippet: For gene silencing experiments, primary LSMCs were transfected with 50 nM of either a non-targeting control siRNA (siNC) or siRNA targeting TDO2 (siTDO2; 5′-CUAUCACUACCUGCGAUCAACUGUG-3′) using PureFection transfection reagent (System Biosciences, Mountain View, CA, U.S.A.), according to the manufacturer’s protocol.

Techniques: Transfection, Control, Gene Expression, Quantitative RT-PCR, Expressing

Journal: Nucleic Acids Research

Article Title: In situ tracking of glycoRNAs on single-cell surface to reveal RNA heterogeneity and transport mechanism

doi: 10.1093/nar/gkag362

Figure Lengend Snippet: SNARE protein knockdown verification and colocalization analysis with glycoRNA. ( A ) Relative mRNA expression of v-SNARE protein after siRNA-mediated knockdown was determined by quantitative real-time PCR (qPCR). ( B ) Protein expression levels of v-SNARE protein were detected by Western blot. ( C ) Quantitative analysis of Western blot result( B ). ( D ) Relative mRNA expression of t-SNARE protein after siRNA-mediated knockdown was determined by quantitative real-time PCR (qPCR). ( E ) Protein expression levels of t-SNARE protein were detected by Western blot. ( F ) Quantitative analysis of Western blot result ( E ). Data are presented as mean ± SD ( n = 3). ( G ) Co-localization of t-SNARE (TSNARE1 channel) and v-SNARE (VTI1B channel) with GLINT-labeled glycoRNAs in the images (FAM channel). ( H ) The plot profiles show co-localization of glycoRNAs with t-SNARE or v-SNARE.

Article Snippet: siRNAs targeting human VTI1B, TSNARE1, and GAPDH, as well as a non-targeting negative control siRNA, were synthesized by Sangon Biotech (Shanghai, China).

Techniques: Knockdown, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Labeling

Journal: iScience

Article Title: Translation factor eIF4G2 directs CD8 + T cell lineage commitment by selectively enabling the IL-7 receptor response

doi: 10.1016/j.isci.2026.115313

Figure Lengend Snippet: eIF4G2 post-transcriptionally sustains γc expression via its mRNA UTRs (A–C) Analysis in primary CD4 + CD8 lo transitional thymocytes. (A) Western blot analysis of γc and IL-7Rα protein levels. (B and C) Quantitative RT-PCR analysis of Il2rg (B) and Il7r (C) mRNA levels ( n = 3, ns p > 0.05, ∗ p < 0.05). (D–H) Mechanistic dissection in 293T cells. (D) Western blot of γc protein in control and EIF4G2 knockdown 293T cells transfected with an IL2RG coding sequence construct containing its native 5′ and 3′ UTRs. (E) Corresponding IL2RG mRNA levels measured by RT-qPCR ( n = 3, ns p > 0.05) . (F and G) Assessment of γc protein stability ( n = 3, ns p > 0.05). (F) Representative western blots and (G) quantification of γc protein levels over time following cycloheximide (CHX) treatment in si-control and si- EIF4G2 293T cells ( n = 3, ns p > 0.05). (H) Western blot of γc protein in si-control and si- EIF4G2 293T cells transfected with an IL2RG CDS construct lacking UTRs. Data are representative of at least two independent experiments. Bar graphs show mean ± SEM and unpaired Students’ t test was used to perform the statistical analysis.

Article Snippet: Cells were transfected either with a non-targeting control siRNA (si-Control) or human EIF4G2 targeting siRNA (si- EIF4G2 ) (Sangon Biotech) using Lipofectamine RNAiMAX transfection reagent (Invitrogen).

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Dissection, Control, Knockdown, Transfection, Sequencing, Construct

Journal: Poultry Science

Article Title: eIF3m promotes fowl adenovirus serotype 4 replication via interacting with ORF1B protein

doi: 10.1016/j.psj.2026.106566

Figure Lengend Snippet: Knockdown of eIF3m suppressed FAdV-4 replication in vitro. (A and B) LMH cells transfected with siRNA targeting at eIF3m or siNC as a negative control were collected at 24 h for qRT-PCR (A) and at 48 h for western blot (B) to evaluate the knockdown effect. (C and D) LMH cells transfected with 100nM/well siRNA or siNC were infected with CH/HNJZ/2015 at an MOI of 0.01. Western blot (C) and virus titration (D) were analyzed to investigate virus growth kinetics. The data shown represent the means±SD, and the experiments were repeated three times. The statistics analysis was performed by 2-way ANOVA. *, P < 0.05, **, P < 0.01.

Article Snippet: Based on the chicken eIF3m sequence obtained from GenBank, three siRNAs along with a non-targeting control (siNC) were synthesized by Shanghai Sangon Biotech.

Techniques: Knockdown, In Vitro, Transfection, Negative Control, Quantitative RT-PCR, Western Blot, Infection, Virus, Titration